A markerless protocol for genetic analysis of Aggregatibacter actinomycetemcomitans
نویسندگان
چکیده
منابع مشابه
Proteomics of Aggregatibacter actinomycetemcomitans Outer Membrane Vesicles
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis and with endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA) into human host cells and to act as triggers of innate immunity upon carriage of ...
متن کاملVirulence Mechanisms of Leukotoxin from Aggregatibacter actinomycetemcomitans
Aggregatibacter actinomycetemcomitans is a gram-negative bacterium that is present in the oral cavity of a large proportion of the human population (Zambon et al., 1983; Henderson et al., 2010) The bacterium is acquired through transmission from infected individuals and thought to initially colonize oral mucosa as a facultative intracellular pathogen (Fine et al., 2006). When the bacteria trans...
متن کاملProteomics of Protein Secretion by Aggregatibacter actinomycetemcomitans
The extracellular proteome (secretome) of periodontitis-associated bacteria may constitute a major link between periodontitis and systemic diseases. To obtain an overview of the virulence potential of Aggregatibacter actinomycetemcomitans, an oral and systemic human pathogen implicated in aggressive periodontitis, we used a combined LC-MS/MS and bioinformatics approach to characterize the secre...
متن کاملHimar1 Transposon for Efficient Random Mutagenesis in Aggregatibacter actinomycetemcomitans
Aggregatibacter actinomycetemcomitans is the primary etiological agent of aggressive periodontal disease. Identification of novel virulence factors at the genome-wide level is hindered by lack of efficient genetic tools to perform mutagenesis in this organism. The Himar1 mariner transposon is known to yield a random distribution of insertions in an organism's genome with requirement for only a ...
متن کاملAntibiotic susceptibility of Aggregatibacter actinomycetemcomitans JP2 in a biofilm
BACKGROUND Localized aggressive periodontitis (LAgP) is an inflammatory disease associated with specific bacteria, particularly Aggregatibacter actinomycetemcomitans, which can result in early tooth loss. The bacteria grow as a biofilm known as subgingival plaque. Treatment includes mechanical debridement of the biofilm, often associated with empirical antibiotic treatment. OBJECTIVE The aims...
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ژورنال
عنوان ژورنال: Journal of the Formosan Medical Association
سال: 2014
ISSN: 0929-6646
DOI: 10.1016/j.jfma.2012.05.005